Cytoskeleton Methods and Protocols by Ray H. Gavin

Author:Ray H. Gavin
Language: eng
Format: epub, pdf
Publisher: Springer New York, New York, NY

2.Remove the bead suspension from the 4 °C rotation apparatus, and warm them up to room temperature.

3.Sonicate the beads for 1 min to break up eventual aggregates and ensure a uniform suspension of single beads.

4.Remove the cell imaging dish from the 37 °C incubator and pipette 1 μL bead suspension onto the cells. Perform this in sterile conditions (see Note 15 ).

5.Return the cells to the 37 °C incubator and let the beads settle for 30 min.

3.3.3.2 Position the Tweezers Over the Cells

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